High Efficiency 5 Min Transformation of Escherichia Coli
نویسندگان
چکیده
منابع مشابه
High efficiency 5 min transformation of Escherichia coli.
We report a simple, rapid, 5 min transformation protocol that produces ∼100% more transformants than the original calcium competent protocol of Mandel and Higa (1). There have been improvements on the transformation efficiency of 105–106 colonies/μg plasmid DNA (2); the best probably that of Hanahan (3) yielding 107–109. The highest rates are achieved using electroporation (109–1010). However, ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/24.3.536